embriones fluorescentes producidos por inyección de espermatozoides incubados con plásmido pCX-EGFP. a: células embrionarias de. inyección intracitoplasmática de espermatozoides. técnica de fertilización. by. nadia vargas. on 10 September Comments (0). Please log in to add your. One of the most innovative techniques in the field of human reproduction is intracytoplasmic sperm injection, where, with important safety and high technology.
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In vitro fertilization IVFthe main treatment for infertility, allows for higher rates of pregnancy than natural conception.
Intracytoplasmic Sperm Injection (ICSI)
Do you need help? We can help you with a no-obligation. This process involves several steps which need to be adequately coordinated. Designing made-to-measure treatment for the patient’s needs, team quality and the clinic’s technology are key to success. Every patient is a unique individual. Our goal is to locate the source of infertility among couples and select the most effective treatment. In the case of women, their cervical canal and endometrial cavity are examined, as well as possible alterations in their fallopian tubes and their menstrual cycle patterns.
If this is not the case, the patients are offered other treatment options. In order to stimulate production of several oocytes and guarantee their quality, we administer a combination of pharmaceutical drugs.
Response is monitored using vaginal ultrasound scans and timely blood analyses: The whole process lasts approximately between 8 and 12 days, depending on each case.
ICSI, Intracytoplasmic sperm injection
The treatment can be cancelled if a low or exaggerated ovarian response is observed. Once the oocytes are mature, we retrieve them with the guidance of a vaginal ultrasound scan under local anaesthesia and light sedation so that it is a completely painless.
This process only takes 15 minutes and does not require an operation, being hospitalised, stitches or the use of general anaesthesia. At the same time, the semen is activated to improve its fertilisation potential. The retrieved eggs are taken into the IVF laboratory where they are prepared for insemination.
IVF: In Vitro Fertilization, treatments at Instituto Bernabeu.
Oocytes and spermatozoids are placed together for several hours within an incubator which intracitoplasmaica the ideal conditions for fertilisation to take place and the subsequent development of pre-embryos.
The number of fertilised eggs will not be known until the next day.
ICSI is performed on oocytes when the reproduction biologist in charge deems it necessary or when it has been previously agreed upon. ICSI proved to be a revolution in assisted reproduction techniques, as it overcomes most male infertility problems. This technique is used intracitoplamatica fertilization when sperm suffers from low count, an abnormal morphology, poor motility or when the sperm is unable to fertilize through IVF.
It can also be used with patients suffering from a blocked sperm duct. In this case, a puncture is made in order to extract intracihoplasmatica directly from the testicles.
This procedure is performed using a microscope. Micromanipulation equipment is also used, allowing us to stabilize the egg softly and subsequently place the sperm inside it. After fertilization, intracitoplasmaticw start to develop in incubators in a culturing environment that provides them with everything they need in order to grow.
They are watched daily, and both embryo cleavage the number of cells and important data about their morphology are recorded. The embryo culture stage can last between two and five days. This enables us to observe the embryo development in order to select the ones with the highest quality. Nature will reject the weaker ones. In general, during a longer culture period, there are fewer viable embryos but they are of much better quality than the embryos achieved during a short culture period. Embryo quality is determined by a combination of cleavage and morphology.
Every embryo faces a different fate. Some have good quality, others become blocked and others make it to the end despite their bad quality. From the way they evolve we propose either short cultures days or long cultures days: Embryo transfer is normally scheduled between two and five days after fertilization i.
The goal is to move the embryo from the laboratory to its final destination for development: It is carried out with the help of an abdominal ultrasound in order to find the perfect spot for implantation. A thin catheter is introduced through the neck of the uterus, and a drop taken from the culturing environment where the embryo is floating is poured into the uterus. Sedation is not necessary, it is not painful and, as a matter of fact, it is very similar to a regular gynecological checkup.
A pregnancy test is scheduled 13 to 14 days after starting to take progesterone to confirm pregnancy or not: This hormone produces the embryo and is transferred to the mother through placenta. In case of failed pregnancy, every member of the human team that participated inyeccio to examine the whole treatment so as to evaluate the causes. Afterwards, a meeting ingeccion the couple is arranged to provide a medical report.
When pregnancy is confirmed and after 15 days, an ultrasound scan is performed to make sure that pregnancy is proceeding normally. To perform it earlier intraciotplasmatica create confusion and uncertainty, as the results are not conclusive in most cases. FIV is a highly flexible procedure. This technique has made it possible for women without a partner or with a same-sex partner Reception of Oocytes from Partner, ROPA to become pregnant. Furthermore, FIV allows patients to take preimplantation genetic diagnosis and this way reduce dramatically the risk of giving birth to children with genetic disorders.
In vitro fertilisation is the most common type of reproductive medicine treatment. Embryos are then transferred in order to facilitate implantation. In terms of disadvantages, it should be pointed out that there is a low risk of complications.
These include ovarian hyperstimulation syndrome and multiple pregnancies. On the whole, patients can return to their daily routine the day after ova extraction or transfer.
The female partner must also generate ova and be free of abnormalities in the uterus. Statistics refer to overall data and should not be extrapolated to any one particular case. It is important to keep in mind that it is essential to always establish a personalised prognosis. This includes transfer of fresh embryos and a possible transfer of cryopreserved embryos when the objective is not achieved in the first transfer.
Please do not intracitoplasmqtica this field unless you are a bot. Subscribe to our newsletter. What is in vitro fertilization?
Ask for a quote. In vitro fertilisation inttacitoplasmatica. IVF using donated ova. IVF using donated semen. Study and preparation of the patients Every patient is a unique individual. Egg retrieval Once the oocytes are mature, we retrieve them with the guidance of a vaginal ultrasound scan under local anaesthesia and light sedation so that it is a completely painless.
Sperm capacitation At the same time, the semen is activated to improve its fertilisation potential.
Laboratory fertilisation The retrieved eggs are taken into the IVF laboratory where they are prepared for inyevcion. Culturing the embryos After fertilization, embryos start to develop in incubators in a culturing environment that provides them with everything they need in order to grow.
Transferring the embryos Embryo transfer is normally scheduled between two and five days after fertilization i. Testing for positive pregnancy A pregnancy test is scheduled 13 to 14 days after starting to take progesterone to confirm pregnancy or not: Pregnancy ultrasound When intracjtoplasmatica is confirmed and after 15 days, an ultrasound scan is performed to make sure that pregnancy is proceeding normally.
Its goal lies in limiting the number of eggs to be retrieved and therefore alleviate the burden of the treatment for the patient without compromising the options accumulated to become pregnant. Much less medication is needed and unlike the classic protocols, not all medication has to be injected. Fewer visits to the clinic are required. Intracitopasmatica produces less discomfort and side effects from ovarian stimulation as it is a lot gentler.
Women under 35 years of age imtracitoplasmatica good ovary reserve and good prognosis. Previous exaggerated ovarian response that should be avoided. Patients at risk of ovarian hyperstimulation.