ANEMIA INFECCIOSA AVIAR PDF

Evaluaci6n del virus de la anemia infecciosa aviar y factores de riesgo aso- ciados con la enfermedad y con las perdidas en producci6n en pollos de engorde. alineacion de las secuencias de la proteina viral 1 de los aislamientos del virus de anemia infecciosa aviar provenientes de Esloven. (CAV/00, CAV/ An outbreak of anemia dermatitis syndrome caused by chicken anemia agent. ( CAA) occurred anemia infecciosa aviar en 15 parvadas de pollos de engorde.

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School of Veterinary Medicine. This is an open access article distributed under the terms of the Creative Commons Attribution License http: This research was conducted on ten glass slides selected from the histopathology evaluation chickens. Histopathology showed severe bone marrow hypoplasia to complete aplasia, fully depletion of the erythrocytic and granulocytic series, both accompanied by space occupying adipocytic replacement. Foci of erythropoietic hyperplasia with intense mielopoietic activity, some hemocytoblast increased of size, with large nucleus.

A quantitative analytical technique by Positive Pixel Count Algorithm was applied. It demonstrated that measures area stained of control slides were higher than CAV slides Average: So, the control slides showed strong positivity, due to the presence of bigger quantity of cells of erythrocytic and granulocytic series.

ANEMIA INFECCIOSA AVIAR by Yezid Alexander Ardila Gomez on Prezi

This technique demonstrates in a quantitative way the severe decrease of the cellular components of the bone marrow in presence of the infection for CAV, supporting with numeric data the histology image evaluated by the pathologist.

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Therefore, it can be used as support to the histopathology of field samples to evaluate the presence of lesions taken place by CAV and this way to improve the quality and efficiency of the veterinary pathology services. The virus causes aplastic anaemia, generalized lymphoid atrophy and increased mortality after infection of day-old susceptible chickens [ 2 ].

The virus causes severe depletion of cortical thymocytes and erythroblastoid cells in the bone marrow, leading to immunodeficiency and anemia [ 3 ], because the VAIA lacks the necessary enzymes to reproduce its DNA in an independent way, it requires the cells to be divided very quickly [ 4 ].

The Positive Pixel Count is a multipurpose algorithm that measures area and intensity of staining for two-colored slides. This technique is useful as support to improve the histopathology evaluation making it objective by means of infecciosx analytical techniques and this way, to improve the quality and efficiency of the pathology services.

The purpose of this study was to apply quantitative analytical technique to histopathology of birds infected experimentally by the virus of chicken anemia virus.

Positive Pixel Count is a multipurpose algorithm that measures area and intensity of staining for two-colored slides.

Virus de l’anèmia del pollastre

Pixels are classified according to colour and intensity of staining. Pixel counts area are calculated for anmia positive Redpositive Orangeweak positive Yellowand negative Blue pixels. This is useful for measuring positivity as a fraction of total stained area. The control slides showed strong positivity, due to the presence of bigger quantity of erythroblastoid cells.

This technique demonstrated in a quantitative way the severe knfecciosa of the cellular components of the bone marrow in presence of the infection for CAV, supporting with numeric data aneemia histology image evaluated by the pathologist. Therefore, it can be used as support to the histopathology of field samples to evaluate the presence of lesions taken place by CAV in the farms and this way to improve the quality and efficiency of the veterinary pathology services.

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This article has been published as part of Diagnostic Pathology Volume 3 Supplement 1, New trends in digital pathology: The full contents of the supplement are available online at http: National Center for Biotechnology InformationU.

Journal List Diagn Pathol v. Published online Jul Author information Article notes Copyright and License information Disclaimer. Supplement New trends in digital pathology: This article has been cited by other articles in PMC.

Abstract This research was conducted on ten glass slides selected from the histopathology evaluation chickens. Open in a separate window.

Conclusion This technique demonstrated in a quantitative way the severe decrease of the anwmia components of the bone marrow in presence of the infection for CAV, supporting with numeric data the histology image evaluated by the pathologist. Acknowledgements This article has been published as part of Diagnostic Pathology Volume 3 Supplement 1, Blue wing disease of chickens: Chicken anaemia virus infection: Isolation and some characteristics of and agent inducing anemia in chicks.

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